Clean room atmosphere for Algae  BIO-MEDICAL R & D.

Fully Automated , 24/7 Algae biomass production, regardless of climate or Latitude 

Because each Fully Automated Photosynthetic Growth Tower (GT) has as many  as 100 shallow growth trays , ( 4m wide x 1.5 back to front  trays x 5 cm deep water growth medium ) within the 2 vertical stacked elevator columns ,  and the GT contains 100 algae bio-mass exposed surfaces, (22) , the growth trays , that are concentrated , the Oxygen produces by the shallow algae's carpet / matt growing on surface (20)  , easily releases its O2 biogases into the air .

The o2 naturally flows up, inside the exterior exoskeleton walls of the GT (21, and the o2 is pulled up and exhausted out of the GT (23), in order that the o2   buildup  does not oxidize the algae production , in each growth tray , by design. 

The o2 naturally  rises vertically,  uP ^ , into top of GT , and o2 is exhausted outside into external ambient air or o2 can be captured at the GT's top mounted exhaust fans.(23)    

One method to see the flow of the oxygen's exhaust path up and through  the fans, would be to put a harmless fluorescent dye into the algae water growth medium ,inside each growth pan, and use a correct light spectrum to illuminate the rising 02 vapor , containing the dye. 

As I read the below problem  described , I do think the GT could be of a benefit , to produce h2 , because of its many layered , redundant controlled conditions, that are monitored by in-situ and ex-situ 02 , h2 , nitrogen , temperature, humidity , and cell count sensors, that are placed inside each growth tray , with real time data continuously being fed back to central computer monitor, to control 24/7 input   , through put and out put of each growth tray. 

Also of benefit , is the "clean room: atmosphere of the GT , as each growth tray's batch harvest occurs at the service window (16) , containing  each completed  algae batch, the growth ray is replaced with a new, fresh , sterilized growth tray, which eliminates the bacterial contamination of the next batch , caused by bacterial buildup on growth container's surfaces, accumulated from the previous algae batch  .

No other algae propagation system can do this.


Prevention of Bacterial Contamination

The ALGAENTIS process of removal and replacement of the Algae Growth Trays, with new sterile growth trays , after each harvest cycle, inserted into the Growth Towers automated  Algae Growth Trays, stacked within the fully automated vertical elevator unit, is what stops the Bacterial Contamination from developing,  from  the previous batch of Algae ,  continuously propagated with the Growth Tower Trays .  Bacterial Contamination , from buildup of algae , within , Vats , Pipes, Open Ponds and Bag systems , is what triggers Bacterial Contamination .  ( as discussed in the recent article on Bacterial Contamination of Algae , reprinted below ; )

Chemical communication between algae and bacteria

December 5, 2017

If green algae of the species Chlamydomonas reinhardtii meet Pseudomonas protegens bacteria, their fate is sealed. The bacteria, measuring only some two micrometers, surround the algae, which are around five times larger, and attack them with a deadly toxic cocktail. The algae lose their flagella, which renders them immobile. The green single-celled organisms then become deformed and are no longer able to proliferate.

The chemical communication mechanism underlying this extremely effective attack has now been uncovered by botanists and natural product chemists at Friedrich Schiller University, Jena (FSU) and the Leibniz Institute for Natural Product Research and Infection Biology — Hans Knöll Institute (HKI).  

It is a gruesome spectacle that meets the eyes of Prasad Aiyar as he looks down the microscope. The doctoral candidate from India, who came to Jena to do his Master’s degree in Molecular Life Sciences, examines the species Chlamydomonas reinhardtii on a microscope slide. The oval-shaped microalgae, a good 10 micrometers in size, have two flagella with which they busily swim around — that is, until Prasad Aiyar uses a pipette to add a drop of a bacterial solution. The even smaller bacteria gather together into swarms, which surround the algae. Just 90 seconds later, the algae are motionless and when one looks more closely, one can see that their flagella have fallen off.

The Jena researchers have discovered why these bacteria have such a devastating effect on the green algae. It seems that a chemical substance plays a central role in the process, as the teams under Prof. Maria Mittag and Dr. Severin Sasso of the FSU, and Prof. Christian Hertweck of the Leibniz Institute for Natural Product Research and Infection Biology — Hans Knöll Institute (HKI) — report in the scientific journal Nature Communications.

Orfamide A, as the substance is called, is a cyclical lipopeptide which the bacteria release, together with other chemical compounds. “Our results indicate that orfamide A affects channels in the cell membrane, which leads to these channels opening,” explains Dr. Sasso. “This leads to an influx of calcium ions from the environment into the cell interior of the algae.” 

A rapid change in the concentration of calcium ions is a common alarm signal for many cell types, which regulates a large number of metabolic pathways. “To be able to observe the change in the level of calcium in the cell, we introduced the gene for a photoprotein into the green algae, which causes bioluminescence if the calcium level increases. This enables us to measure the amount of calcium with the help of the luminescence,” explains Prof. Mittag, Professor for General Botany. In some cases, the changes in the calcium lead to changes in the direction of movement, for example, after light perception. In other cases, for example after the bacterial attack, they cause the loss of the flagella.

In addition, the teams were able to show that the bacteria can tap the algae and use them as a nutrient source if they are lacking in nutrients. “We have evidence that other substances from the toxic cocktail released by the bacteria also play a role in this,” says Maria Mittag. Her team, once again in cooperation with the teams of Prof. Hertweck und Dr. Sasso, now also wants to track down these substances, in order to gain a precise understanding of this chemical communication between algae and bacteria. 

Numerous research groups have dedicated their efforts to studying the “chemical language” between microorganisms and their environment as part of the Collaborative Research Centre “ChemBioSys.” Microbial species communities occur in virtually every habitat on Earth. “In these communities, both the species composition and the interrelations between individual organisms of one or more species are regulated by chemical mediators,” says Prof. Hertweck, who is the speaker for the Collaborative Research Centre and head of the Biomolecular Chemistry department at HKI. 

The aim of the interdisciplinary research partnership is to explain the fundamental control mechanisms in complex biosystems, which affect the whole of life on Earth. “We want to understand the mechanisms through which the microbial community structures are formed and their diversity maintained. In view of the huge significance of microalgae for human life, we still know astonishingly little about the fundamental elements and the interactions in their microscopic world,” says Prof. Mittag.


The ALGAentis Biosolutions' fully automated algae cultivation process also delivers a new “clean room” platform ,  for the emerging Algal Bio-Medical field to scale up within , post lab.



Clean Room

Photo- Bio-Reactor


Contamination of Algae spore crops.



  growth Processors: